Hsp70 Rna Expression in Rainbow Trout (oncorhynchus Mykiss) Clonal Lines
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چکیده
By Pilar Heredia-Middleton Washington State University December 2005 Chair: Gary Thorgaard Several review articles have been published on the role of heat shock proteins, and their link to temperature in fish and other organisms. Heat shock protein 70 (70KDa) is the most commonly-expressed protein in response to thermal stress, and its expression has been associated with differences in thermal habitats. This thesis presents a general background and review of heat shock proteins, followed by a review of aquatic and marine organisms’ response to thermal stress. In addition, I described the heat shock response to thermal stress of Oncorhynchus mykiss rainbow trout clonal lines; developed from individuals from different habitats. The experiment characterized response of three clonal lines, Arlee, OSU and Whale Rock. The expression patterns of hsp70 RNA from blood were quantified using real-time PCR. The hsp70 RNA expression patterns of these clonal lines were found to be significantly different. The most striking result was the lack of any detectable response by the Arlee line. Both Whale Rock and OSU hsp70 expression patterns showed a maximum induction temperature at 22°C; Whale Rock
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